|
Document Type |
: |
Thesis |
|
Document Title |
: |
Phytochemical and Biological Studies of Cucumis prophetarum دراسات كيميائية و بيولوجية على نبات شري الذئب |
|
Subject |
: |
faculty of Pharmacy |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Phytochemical study of the MeOH extract of Cucumis prophetarum L. fruits (Family Cucurbitaceae) using different chromatographic techniques led to isolation of three the metabolites; spinasterol (1), cucurbitacin B (2) and 2-O-β-D- glucopyranosylcucurbitacin E (3). Their chemical structures were established on the basis of physical, chemical, spectral data 1D (1H and 13C NMR) and 2D NMR (HSQC and HMBC) as well as comparison with literature data. Cucurbitacin B (Cu-B) (2) was found to be the major constituent. The potential protective activities of MeOH extract, CHCl3 and EtOAc fractions and Cu-B were evaluated against carrageenan-induced prostatic inflammation in rats. Acute toxicity was assessed by evaluating the LD50. Pre- treatment with the CHCl3 fraction and Cu-B ameliorated the rise in prostate index and obviously protected against histpathological changes. Further, the MeOH extract, CHCl3 and EtOAc fractions as well as Cu-B significantly protected against oxidative stress in prostatic tissues. The anti-inflammatory activities of the extract, fractions and Cu-B were confirmed by ameliorating the rise in prostatic content of the inflammatory mediators TNF-α, IL-1β, COX-2 and iNOS induced by carrageenan. In addition, the rise in the chemotactic factors MPO, F4-80 and MCP-1 was significantly hampered. In conclusion, three known compounds (1-3) were isolated from Cucumis prophetarum L. fruits. Cu-B (2) was the major identified compound. Particularly, CHCl3 fraction and isolated Cu-B exhibited potent anti-inflammatory activity against carrageenan-induced prostatitis. The anti-inflammatory activity can be attributed, at least partly, to inhibition of neutrophil and macrophage infiltration into prostatic tissues. |
|
Supervisor |
: |
Prof. Dr. Ibrahim Attia Shehata |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1440 AH
2019 AD |
|
Co-Supervisor |
: |
Prof. Dr. Gamal Abdallah Mohamed Hussein |
|
Added Date |
: |
Wednesday, August 21, 2019 |
|
Researchers
| عمر سعد الجهني | Aljohani, Omar Saad | Researcher | Master | |
|