Document Details
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Document Type |
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Thesis |
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Document Title |
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Chemical Characterization of Some Red Sea Species Belong to the Alcyonacea التوصيف الكيميائي لبعض المراجين الرخوة من البحر الأحمر |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Four new pyran-based cembranoid diterpenes (194, 195, 197 and 199) along with ten known compounds including four sesquiterpenoids (1,2,193,196), five diterpenoids (21, 38, 62, 80, 81), and a C30 steroid gorgosterol (198) were isolated from the Red Sea soft-bodied coral Sarcophyton glaucum. Sarcoglauphine A (194) and B (195) are conformers around the single bond C-6/C-7, while sarcotrocheliol-B acetate (199) and sarcotrochelione (197) are functional isomers at C-7. The chemical structures of the isolated compounds were obtained by extensive interpretation of the measured spectroscopic spectra. The sesquiterpenoid compound naphthalene, 1, 2-dihydro-4,7-dimethyl-1-(1-methylethyl) (196) was isolated for the first time from Sarcophyton sp., this could be as chemotaxonomy marker for Sarcophyton. The cytotoxicity of compounds (194, 195, 197, 199) on viability of HepG2, MCF-7, and HCT116 cell lines was assessed by Sulphorhodamine B (SRB) method. Doxorubicin was used as positive control and its mean IC50 against HepG2, MCF-7, and HCT116 cells was 3.5, 2.4 and 6 µM, respectively. The obtained results showed that most of the activity was observed for compound (194) with mean IC50 of 60, 49.9 and 35 µM and compound (195) with mean IC50 of 57.1, 54.07 and 35.82 µM against HepG2, MCF-7 and HCT116 cell lines, respectively. The crystal and molecular structure of sarcotrocheliol (81) was determined using single crystal X-ray and NMR methods. Crystallography showed that the molecule is crystalline as a monoclinic, space group of P212121, with the a = 9.20(4) Å, b = 10.80(4) Å, c = 19.99(9) Å. 1H, 13C and DEPT-135 NMR measurements of sarcotrocheliol are reported in four different deuterated solvents; CDCl3, CD3CN, MeOD4 and DMSO-d6. |
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Supervisor |
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Prof. Khalid O. Al-Footy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Walied M. Alarif |
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Added Date |
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Tuesday, June 13, 2017 |
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Researchers
| زهراء عبد الكريم السيهاتي | Alsaihati, Zahra Abd ulkareem | Researcher | Master | |
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