Document Details

Document Type : Thesis 
Document Title :
Detection of Glucokinase Gene Mutation in
الكشف عن طفرات جين الجلوكوكيناز في سيدات سعوديات مصابات بسكري الحمل
 
Subject : Chemistry department 
Document Language : Arabic 
Abstract : Gestational diabetes is a condition in which pregnant women exhibit elevated blood glucose levels especially during the third trimester. It is usually caused because the body does not secrete enough insulin to meet the body’s needs. Glucokinase (GCK) enzyme plays a significant role in glucose homeostasis and promotes insulin release from pancreatic beta cells. Mutation in the GCK gene leads to various diseases. It has already been well established that GCK mutations are associated with development of many types of diabetes. For this, we screened exons 7, 8 and 9, which are specific for pancreatic glucokinase, for mutations at posi¬tions 682A>G, p.T228A, 895G>C, p.G299R and 1148C>A, p.S383X, respectively in pregnant women with or without gestational diabetes in a Saudi population. A sample of 100 pregnant women classified as healthy (control, N = 50) or with gestational diabetes (N = 50) was analyzed for mutations in the GCK gene. The target part of GCK gene was isolated and amplified by the polymerase chain reaction (PCR). PCR products were digested by restriction enzymes: BstUI for exon 7, HhaI for exon 8 and BfaI for exon 9, and electrophoresed on agarose gel. Mutations were detected by restriction fragment length polymorphism. None of these GCK mutations were found to be significantly associated with gestational diabetes mellitus. We conclude that mutations in GCK gene do not affect glycemic control and are not correlated with gestational diabetes mellitus in Saudi Population 
Supervisor : Dr. Archana P. Iyer 
Thesis Type : Master Thesis 
Publishing Year : 1435 AH
2014 AD 
Co-Supervisor : Dr. Fahad Al-Abbassi 
Added Date : Monday, July 21, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
صباح محمد حسانHassan, Sabah MohammedResearcherMaster 

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