Document Details
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Document Type |
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Thesis |
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Document Title |
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MOLECULAR IDENTIFICATION, ECOLOGICAL AND CONTROL STUDIES ON DENGUE FEVER VECTOR, AEDES AEGYPTI (L.) IN JEDDAH -SAUDI ARABIA دراسات في التعريف الجزيئي وبيئة ومكافحة الناقل الحشري لحمى الضنك Aedes aegypti في محافظة جده – المملكة العربية السعودية |
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Subject |
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Department of Arid Land Agriculture |
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Document Language |
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Arabic |
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Abstract |
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Adults and immature stages of the dengue fever vector Aedes aegypti were identified based on morphological characters using light and electron microscope and appropriate illustration (pictorial) keys. Molecular identification were conducted by isolating DNA and protein analysis of Ae. aegypti. The results showed that DNA could be isolated from mature and immature stages of Ae. aegypti in the laboratory and from older and/or field specimens. This assay consisted of different primers reaction, which could amplify the DNA of both mature and immature stages producing fragment of three distinct sizes, ~1250 bp , 500 bp and 300~ 400 bp, respectively .Protein samples of (larva, pupa and adult) for lab and field strains of dengue fever vector, Ae. aegypti were isolated. Proteins were analyzed using sodium dodecyl sulfate polyacrylamide (SDS-PAGE).The SDS-PAGE analysis showed that, a total of 12 protein bands were detected. The relative molecular weight of the detected bands was approximately in the range of 16.6 – 75.6 kDa.
House-to-house surveys of larval population of Ae. aegypti were conducted to determine the importance of house index of difference habitats in Jeddah governorate. The statistical analysis showed that the presence of larval stages of Ae. aegypti recorded throughout the year inside houses of the studied locations of (Ghuleel, Al-Balad, Al-Jameiah, Al-Nazlah Al-Yamaneyyah, and Al-Safa).). House index (HI), Container index (CI), and Breteau index (BI) were used to monitor the infestation levels of Ae. aegypti larvae. House indices of each locality was compared with the highest ratio of standard WHO (5- 10%) was as follows, 8.70%, 7.60, 6.60, 6.22 and 4.00% respectively (P < 0.05). Significant increase in the density of larvae was found during November, March., June and January.
The seasonal abundance of adult mosquitoes in Jeddah city was investigated using light traps (Black Hole) and manual methods were used for collecting adults and immature stages in five residential localites in Jeddah .Total 796 adults of Aedes aegypti mosquitoes were collected, out of these, 373 were male (46.8 %) and, 423 females (53.2%). Out of 950 collected immature stages, there were 83.41 % larvae whereas pupae were 16.6%. The statistical analysis showed that both adult and immature stages of Ae. aegypti were present throughout the year, but with different densities depending on the prevailing climatic conditions and the effectiveness of the breeding sites.
Biological effects of non-conventional insecticides, Sumilarv ( pyriproxyfen 0.5 WP ) and Flubex (diflubenzuron DT 6%) against the mosquito larvae of Ae. aegypti (L.) have been evaluated. The mortality percentage of mosquito larvae ranged from 10-38% and 5-18% for diflubenzuron and pyriproxyfen respectively.
The larvicidal activity of crude acetonic extracts derived from leaves and peels of Lantana camara , Ruta chalapensis, Rhazya stricta and Punica granatum where larval mortality percentage inhibition of mosquito ranged 25-97,23-94 ,17-96 and 10-72 % respectively. The late percentage effectiveness of adult emergence, of mosquito ranged in 26-100,13-100 ,10-100 and 22-92 % respectively. |
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Supervisor |
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Prof. Dr. Ahmed Ali Zaitoun |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Co-Supervisor |
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Prof. Dr. Khalid Mohamed Al - Ghamedi . Prof. Dr. Mohamed Abdul-Raheem Shaheen |
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Added Date |
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Sunday, June 9, 2013 |
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Researchers
| عباس محمد العزب | Al-Azab, Abbas Mohammed | Researcher | Doctorate | |
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