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Document Type |
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Thesis |
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Document Title |
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Bioactivity Evalualion of Nalural Honey bee Products and Plant Extracts on Some Plant Palhogens تقييم النشاط الحيوي لكل من المنتجات الطبيعية لنحل العسل والمستخلصات النباتية على بعض الممرضات النباتية |
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Subject |
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Department of Arid Land Agriculture |
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Document Language |
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Arabic |
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Abstract |
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Bacterial plant diseases caused heavy yield and growth losses to agricultural crops worldwide. The excessive use of chemical pesticides to control the bacterial plant diseases cause environmental and public health risks and disturb the ecological balance of beneficial organisms, in addition to the high economic cost. Among the most important bacterial plant diseases are soft rot , Erwinia cartovora and crown gall bacteria , Agrobacterium tumefaciens which cause enormous damage to vegetable crops and fruit. The applic alion of chemicals to control both diseases affecting environment and consumers health. Therefore, altern alive methods to control these diseases are urgently need. This study was conducted in the labor alory of plant protection, department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid land Agriculture. The present study aims to use altern alive methods to control soft-rot and crown gall diseases. Firstly, study the effects of applying n alural products of honeybees, such as honey – Royal Jelly and bee gum(Propolis) to control soft-rot and crown gall diseases al the lab level and subsequently in greenhouses. Secondly, study the effects of some extracts of plants grown in Saudi Arabia, such as Shazab – Lantana camara –Pomegran ale (pomegran ale peel) and Arak on these diseases. Several concentr alions from these plant extracts were tested with three replic ales each to control both diseases al lab level. The highly performed plant extracts were tested on infected plants in the field. We prepare a number of discs with diameter of 0.7cm using a mincing machine paper from filter paper type W altman No. 1. Then collected these discs in a glass bottle sealed and sterilized until using. The center of nutrient agar was inocul aled with colonies of the bacteria and distributed uniformly and left the dishes for 15 minutes. After th al disks was putted by sterilized forceps in specific places in the dish were it was put in the dish four sterile discs and marked withfrom outsides. The concentr alion of plant extracts and honey bee products were applied on the discs using sterile Micropipette. Dishes were incub aled al 30C for 48 hours and then results were recorded with measuring average inhibition zone. The results showed th al Lantana camara with the concentr alion of 9000 and 8000 ppm was the highest inhibition zone of the bacteria with average of inhibition zone of (1.303, 1.227 cm , respectively) which is highly significant. While the highest results was showed in case of crown gall for tre alment with Shazab al the concentr alion of 9000ppm with average of inhibition zone of 1.210 cm. Also , the concentr alion of 9000 ppm of pomegran ale peel give 1.197 cm inhibition zone with no significant differences between two plant extracts.
As for honey products, the results showed th al royal jelly with the concentr alion of 9000 ppm with the highest for inhibition of soft rote bacteria with average inhibition zone of 1.403 cm. As for grown gall bacterial , the highest transaction in their effect on inhibition of bacteria is the Propolis concentr alion of 9000 ppm with an average (1.242 cm) followed by a honey concentr alion of 8000 ppm with an average of 1.230 cm.
Field applic alion had confirmed th al all of Propolis and Lantana camara in addition of honey were the highest for inhibition of growth of the plant p alhogenic bacteria with the presence of significant differences between them and other tre alments. The results of the field experiment as follow: 97.72-9.35-854.16 and 87.39-7.50-775.76 (length- diameter and leaf area) for Propolis and Lantana camara , respectively.
These plant extracts and honey bee products will be a promised altern alives to control the bacterial diseases in vegetable crops to avoid the use of chemical pesticides with harmful effects to environment and health. |
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Supervisor |
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Prof. Dr. Ahmed Ail Zaitoun |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Co-Supervisor |
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Prof. Dr. Mohamed Hassan Madkour |
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Added Date |
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Monday, July 9, 2012 |
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Researchers
| نجيب مرعي المسعودي | ALMASOUDI, NAJEEB MAREI | Researcher | Master | |
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