Document Details Document Type : Article In Journal  Document Title : The transport of glutamine and alanine into rat colonocytes The transport of glutamine and alanine into rat colonocytes   Subject : Biochemistry  Document Language : English  Abstract : The transport of glutamine and alanine into isolated rat colonocytes was studied. The transport of both amino acids appears to be dependent on a Na+ gradient. The apparent Km values for the transport of glutamine and alanine were 2.56 +/- 0.84 and 5.35 +/- 1.20 mM respectively, but with similar Vmax. values. Glutamine and alanine transport were mutually competitive, and the transport of both amino acids was competitively inhibited by 2-methylaminoisobutyrate. In contrast, histidine inhibited the transport of both glutamine and alanine non-competitively. It is concluded that glutamine and alanine are transported into rat colonocytes by a common carrier system similar to System A of other cells. It is suggested that the metabolic function of this transport system in rat colonocytes might be the partial exchange of extracellular glutamine for intracellular alanine  ISSN : 0264-6021  Journal Name : The Biochemical Journal  Volume : 238  Issue Number : 1  Publishing Year : 1406 AH 1986 AD  Article Type : Article  Added Date : Monday, November 28, 2011  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email محمد العرضاوي Ardawi, Mohammed Researcher Doctorate   Files File Name Type Description  31397.pdf pdf   Back To Researches Page